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活性蛋白
- Recombinant Human B-cell receptor CD22(CD22),partial (Active)
- 恒遠(yuǎn)生物蛋白表達(dá)平臺(tái)建立了從原核到真核四大重組表達(dá)系統(tǒng),并建立無細(xì)胞表達(dá)系統(tǒng),擁有基于昆蟲病毒外膜蛋白展示的蛋白表達(dá)技術(shù)、膜蛋白表達(dá)技術(shù)等一系列蛋白表達(dá)及純化技術(shù),有能力制備各種高難度蛋白。
Recombinant Human B-cell receptor CD22(CD22),partial (Active)
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貨號(hào)：HYB-100156
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規(guī)格：
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圖片：
產(chǎn)品詳情
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純度：Greater than 94% as determined by SDS-PAGE.
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內(nèi)毒素：Less than 1.0 EU/ug as determined by LAL method.
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生物活性：Measured by its binding ability in a functional ELISA. Immobilized CD22 at 2 μg/ml can bind Anti-CD22 rabbit monoclonal antibody, the EC50 of human CD22 protein is 4.034-4.800 ng/ml.
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基因名：CD22
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別名：B cell receptor CD22 precursor; B lymphocyte cell adhesion molecule; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL CAM; BL-CAM; BLCAM; CD 22; CD22; CD22 antigen; CD22 molecule; CD22 protein; CD22_HUMAN; Lectin 2; Leu14; Lyb8; MGC130020; sialic acid binding Ig like lectin 2; Sialic acid binding immunoglobulin like lectin 2; Sialic acid-binding Ig-like lectin 2; SIGLEC 2; Siglec-2; SIGLEC2; T cell surface antigen Leu 14; T-cell surface antigen Leu-14
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分子結(jié)構(gòu)：
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種屬：Homo sapiens (Human)
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蛋白長(zhǎng)度：Partial
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來源：Mammalian cell
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分子量：77.9 kDa
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表達(dá)區(qū)域：20-687aa
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氨基酸序列DSSKWVFEHPETLYAWEGACVWIPCTYRALDGDLESFILFHNPEYNKNTSKFDGTRLYESTKDGKVPSEQKRVQFLGDKNKNCTLSIHPVHLNDSGQLGLRMESKTEKWMERIHLNVSERPFPPHIQLPPEIQESQEVTLTCLLNFSCYGYPIQLQWLLEGVPMRQAAVTSTSLTIKSVFTRSELKFSPQWSHHGKIVTCQLQDADGKFLSNDTVQLNVKHTPKLEIKVTPSDAIVREGDSVTMTCEVSSSNPEYTTVSWLKDGTSLKKQNTFTLNLREVTKDQSGKYCCQVSNDVGPGRSEEVFLQVQYAPEPSTVQILHSPAVEGSQVEFLCMSLANPLPTNYTWYHNGKEMQGRTEEKVHIPKILPWHAGTYSCVAENILGTGQRGPGAELDVQYPPKKVTTVIQNPMPIREGDTVTLSCNYNSSNPSVTRYEWKPHGAWEEPSLGVLKIQNVGWDNTTIACAACNSWCSWASPVALNVQYAPRDVRVRKIKPLSEIHSGNSVSLQCDFSSSHPKEVQFFWEKNGRLLGKESQLNFDSISPEDAGSYSCWVNNSIGQTASKAWTLEVLYAPRRLRVSMSPGDQVMEGKSATLTCESDANPPVSHYTWFDWNNQSLPYHSQKLRLEPVKVQHSGAYWCQGTNSVGKGRSPLSTLTVYYSPETIGRR
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蛋白標(biāo)簽：C-terminal 6xHis-tagged
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產(chǎn)品提供形式：Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液：Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
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儲(chǔ)存條件：Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項(xiàng)：Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
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產(chǎn)品描述：
The human B-cell receptor CD22 mediates the interactions of the B-cell and the B-cell antigen receptor signaling. It responds to sialylated glycoproteins and can be affected by free sialic acid. Its function is the regulation of cell adhesion, immunoglobulin secretion, and B-cell proliferation. The recombinant protein is the 20-687aa region of the CD22, expressed in mammalian cells. The protein was fused with a 6xHis-tag on the C-terminus, with a molecular weight of 77.9 kDa. SDS-PAGE analysis shows that the product has a purity greater than 95%. As well, it was determined by ELISA that the EC50 was 4.034-4.800 ng/ml for binding to the Anti-CD22 rabbit monoclonal antibody. The final product has low levels of endotoxin, with less than 1.0 EU/μg as determined by the LAL method. The protein can be used on binding and modulation essays; this with the scope to determinate novel antigens presented by IgM, B-cell differentiation, and modulation and immunoglobulin production studies. It can be used as well in cancer research on tumoral antigen response and modulation.
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Datasheet & COA：Please contact us to get it.
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